Hello xferior-ga,
A paper from Volume 3 of the Journal of the Professional Association
for Cactus Development (JPACD) published in 1998.
“Establishment and Transformation of Callus and Cell Suspension
Cultures of the Prickly-Pear (Opuntia ficus-indica)” from Institute
for Biochemistry and Plant Virology in Braunschwieg, Germany, and
University of Ceara, Brazil (full text at
http://server3003.freeyellow.com/jpacd/llamoca.htm )
used a medium based on the MS medium developed for tobacco plant cell
culture ((Murashige, T., and F. Skoog. 1962. A revised medium for
rapid growth and biossays with tobacco tissue cultures. Physiol.
Plant. 15:473-497):
“friable callus medium (FCM), consisting of full-strength MS salts and
vitamins (Murashige and Skoog, 1962), 0.2 mg/l 6-furfurylaminopurine
(K), 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 0.25 mg/l
4-amino 3,5,6-trichloropicolinic acid (Pickloram), 400 mg/l casein
hydrolysate, 3% sucrose and 0.8% agar. All media were adjusted to pH
5.8 with 1M KOH and autoclaved at 121°C (2 kg/cm2) for 15 minutes.”
This was used for induction of friable callus, and the same medium,
but without the agar was used for cell suspension cultures.
A full recipe for the preparation of the MS medium is available at:
http://user.school.net.th/~anuparp/media2.htm (Chitralada School's
Plant Tissue Culture Laboratory)
Another paper entitled “Somatic Embryogenesis in Some Cactus and Agave
Species from Centro de Investigacion y Asistencia en Tecnologia y
Diseno del Estado de Jalisco, A. C., División de Micropropagacion y
Mejoramiento Genetico Vegetal, Guadalajara, Jalisco, Mexico. (full
text at http://server3003.freeyellow.com/jpacd/santcruz.htm ), also
published in Volume 3 of the Journal
of the Professional Association for Cactus Development (JPACD) in
1998,
describes a number of culture media, also mostly based on the one
developed by Murashige and Skoog
Search strategy: 1. “culture medium” cactus; 2. “MS medium” |
