I'm afraid your research requires more time than has been allowed and
effort greater than that usually associated with the fee you have
proposed.
So, here are a few leads that might be helpful. They are simply
citations that may lead to to further information.
Dr. Francis Collins (a University of Michigan geneticist who now
directs the National Human Genome Research Institute developed a
technique, called chromosome jumping, which allows researchers to
travel along the chromosome at a faster pace while seeking the
genetic mutation responsible for Cystic Fibrosis
http://www.scienceshorts.com/Lap-CheeTsui.html
Rommens, et al (1989) Identification of the cystic fibrosis gene:
Chromosome walking and jumping. Science 245: 1059-1065.
1985
Louis Kunkel, Ph.D., at Harvard Medical School used
a technique called "chromosome walking" to find that these patients' X
chromosomes were missing a huge gene
Chromosome walking is a process by which researchers clone
DNA starting from a particular marker of known sequence and obtain
overlapping fragments of contiguous DNA which hopefully contains the
disease gene. These overlapping cloned DNA fragments can be can be
sequenced and analyzed to determine the presence of genes. Chromosome
walking was a method that was already available but that had some
disadvantages. It was a solid technique for finding short regions of
DNA and could be helpful in locating disease genes that were close to
the markers, but if the markers were too far away, it simply was not a
viable option. Additionally, chromosome walking could easily be
stopped by unclonable sections of DNA, which would hinder the process
of obtaining a copy of the desired segment (Rommens, et al., 1989).
A solution to this problem was achieved with the advent of
chromosome jumping (Marx, 1989), which allows the skipping of regions
of DNA, including those troublesome unclonable sections as discussed
above. As well, chromosome jumping could be used to jump from distant
markers towards the gene of interest; thus, with chromosome jumping,
very near markers were not as essential as they had been before, as
whole regions can be skipped. Chromosome jumping allowed researchers
to “jump” from RFLPs in either direction, and from that jump, a
bidirectional walk could be initiated to find the disease gene
(Rommens, et al., 1989).
http://dragon.zoo.utoronto.ca/~J02T0101C/research.htm#Chromosome
Walking and Jumping
Chromosome walking is a very powerful technique for isolating a gene
responsible for a phenotype without prior knowledge of the gene or its
product. The strategy in chromosome walking is to map the gene of
interest down to a small segment of a chromosome and then stepwise
arrange a series of overlapping clones until the gene is cloned. This
series of overlapping clones could for example consist of Bacterial
Artificial Chromosomes, BAC, which are circular vectors for Eschericia
coli that can carry up to 400 kb
http://www.plantae.lu.se/fskolan/arabidopsistexter/ClasTomasLarsson.html
Monaco. A. P., Larin, Z. (1994), YACs, BACs, PACs and MACs: artificial
chromosomes as research tools, Tibtech (12) 280-286.
Shizuya, H., Birren, B., Kim, U.-J., Mancino, V., Slepak, T., Tachiri,
Y., Simon, M. (1992), Cloning and stable maintenance of
300-kilobase-pair fragments of human DNA in Eschericia coli using an
F-factor-based vector , Proc. Natl. Acad. Sci. USA (89) 8794-8797.
Rommens JM, Iannuzzi MC, Kerem BS, Drumm ML, Melmer G, Dean M,
Rozmahel R, Cole JL, Kennedy D, Hidaka N, Zsiga M, Buchwald M, Riordan
JR, Tsui LC and Collins FS (1989) Identification of the cystic
fibrosis gene: chromosome walking and jumping. Science 245:1059-1065.
After looking at material available on the Internet, I believe you
might have more luck with periodicals search. "Nature" and "Science"
may be two good places to start.
Good luck.
Nellie Bly
Google Answers Researcher |
