Google Answers: Biochemistry, chromatin

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Q: Biochemistry, chromatin ( No Answer, 0 Comments )

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Subject: Biochemistry, chromatin
Category: Science > Biology
Asked by: cooonger-ga
List Price: $5.00

Posted: 04 Jun 2003 12:47 PDT
Expires: 04 Jul 2003 12:47 PDT
Question ID: 213073

I need to know the total charge density of the human histone octamer that DNA
wraps around to form chromatin.

Request for Question Clarification by synarchy-ga on 04 Jun 2003 18:46 PDT

Hello - let me know if this information is along the lines of what
you're looking for:

The histones that comprise the core octamer are two copies each of
histone 2a, 2b, 3, and 4.  The net charges for each protein are given
as:

2a - +15
2b - +19
3 - +20
4 - +16

The overall charge for the octamer is therefore +140.
http://medweb4.bham.ac.uk/jspdf/m102pdf/DNA1.pdf

For more complex methods of calculating the surface charge density,
the following reviews are available:
http://www.aip.org/pt/vol-53/iss-9/p38.html
http://arxiv.org/PS_cache/cond-mat/pdf/0110/0110032.pdf
http://monod.biomath.nyu.edu/index/papdir/fulllengths/pap_2_71.pdf
http://home.uchicago.edu/~ntt/papers/JCP115_7298.pdf

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