Hi dafna,
I presume that you want to know if soy isoflavones have been
extracted before 1995. A bit of researching indicated that the first
extraction was more than 70 years ago.
It has been known since 1931 that soybeans contain relatively high
concentrations of isoflavones (Walz 1931); genistein glycoside was
first isolated from soybeans almost 60 years ago (Walter 1941). An
appreciation of the hormonal potency of isoflavones became apparent
with the recognition in the mid-1940s that an infertility syndrome in
sheep was caused by the ingestion of clover containing high levels of
the related isoflavones formononetin and biochanin A (Bennetts et al.
1946). These two methoxylated isoflavones were metabolized by
intestinal bacteria to equol, a unique mammalian isoflavone that shows
much greater affinity for binding to estrogen receptors than do the
clover-derived isoflavones.
( Source :: http://www.soyfoods.com/3rdSoySymp/AbsorptionAndMet.html )
The above site has a lot of information on the subject you're looking
for.
Isoflavones occur predominantly as glycosides in plants and
consequently are highly polar (water-soluble) compounds (Walz 1931[]
). Comprehensive analyses of the isoflavone content of numerous soy
foods have been reported and generally indicate that most contain
0.1–3.0 mg/g of total isoflavone (Coward et al. 1993[] , Murphy 1982[]
). Soy germ products derived from the hypocotyledon provide one of the
most concentrated (>20 mg/g) sources of isoflavones.
( Source :: http://www.annieappleseedproject.org/sourofisab.html )
I hope this answer has been helpful. If not, please ask for a
clarification, and be a bit more specific. I'm not happy unless you
are. Thank you, and have a good day.
Cheers,
aditya2k. |
Clarification of Answer by
aditya2k-ga
on
17 Jun 2002 15:29 PDT
Hi dafna,
Since you mentioned patent infringement, I browsed through the
patent database and found the following documents :
Process for obtaining genistin malonate and daidzin malonate
http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5141746
Abstract
Genistin and daidzin malonates are obtained by extracting ground soya
bean with methanol or ethanol to obtain an extract. The extract is
buffered to a pH of 6 to 9 and then extracted with a water-immiscible
solvent. The aqueous phase is recovered, acidified to a pH of 2 to
5.4, and then extracted with a water-immiscible solvent. The organic
phase is recovered, neutralized to a pH of 6.8 to 7.2, and the
malonates are separated therefrom.
Aglucone isoflavone enriched vegetable protein fiber
http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&u=/netahtml/search-adv.htm&r=62&f=G&l=50&d=PALL&s1=glycitein&p=2&OS=glycitein&RS=glycitein
Abstract
The present invention relates to an aglucone isoflavone enriched
vegetable protein fiber wherein a vegetable protein material is
extracted to form a slurry of protein, fiber and glucone isoflavones.
The pH of the slurry is adjusted to about 6 to 8 and the slurry
reacted with a beta glucosidase to convert the glucone isoflavones in
said slurry to aglucone isoflavones. The fiber fraction is then
recovered from the slurry by centrifugation or similar means to
provide an aglucone enriched fiber.
If you could provide me with the name of all the 12 isoflavones, I
could provide more information to you in a shorter period of time.
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