Hi smitty3360,
In a word, No. Not directly. You can, however get to a pure culture,
but you must go through several steps.
If you have a mixed broth, (or a mixed slant culture) your first step
will be to take a loopful of the broth (or, from a slant, a straight
loopful of colonies that resemble the ones you are hoping to isolate)
and streak it for isolation onto various types of agar, including
non-selective agar such as sheep blood agar, and some selective media
such as Thayer-Martin, MacConkeys, PEA, HE, etc. The kind of organism
you are hoping to isolate will determine what type of selective media
you will use. Most all types of organisms will grow on blood agar,
while MacConkeys?s will inhibit growth of gram positive organisms,
allowing gram negative organisms to grow, making for easier isolation.
Once this first culture has grown out, you will select, from
experience and recognition, a few colonies of the organism wanted for
a pure culture. A loopful of this colony is then streaked for
isolation onto the appropriate media and a new broth or slant.
When this subculture has grown, you will have a pure culture. A gram
stain will confirm this. Each laboratory has its own set of testing
methods and favorite selective media for creating a pure culture. Some
may use antibiotics to inhibit growth of unwanted organisms.
With a slant, you actually have a better chance of getting a pure
culture quicker-most slants are selective media, and you can see the
colonies from which you wish to obtain a pure culture. This enables
you to select colonies that best match the species you are hoping to
isolate.
That?s it in a loopful!
If any part of my answer is unclear, please request an Answer
Clarification before rating. This will enable me to assist you
further!
Regards,
crabcakes
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