All methods will need a validation for your sample matrices.
Type Pubmed at Google search screen (should be first link)
At Pubmed enter: glycerol AND method AND analysis (53 results)
sort thru yielded:
Analytical Biochemistry 2000 Aug 1;283(2):192-9
Determination of carbohydrates, sugar alcohols, and glycols in cell cultures and
fermentation broths using high-performance anion-exchange chromatography with
pulsed amperometric detection. Hanko VP, Rohrer JS.
Dionex Corporation, 500 Mercury Drive, Sunnyvale, California, 94088-3603, USA.
val_hanko@dionex.com
Cell cultures... We used high-performance anion-exchange
chromatography with pulsed amperometric detection (HPAE-PAD) to simultaneously
analyze for carbohydrates, alditols, and glycerol in growing yeast
(Saccharomyces cerevisiae) cultures and their final fermentation broths...
PMID: 10906239 [PubMed - indexed for MEDLINE]
2: Journal Chromatograph Science 2000 Jun;38(6):259-61
A simple high-performance liquid chromatography method for the analysis of
glucose, glycerol, and methanol in a bioprocess.
Parpinello GP, Versari A.
Dipartimento di Biotecnologie Agrarie ed Ambientali, Universita di Ancona,
Italy.
A reliable high-performance liquid chromatography-refractive index detection
method for the simultaneous analysis of glucose, methanol, and glycerol in a
bioprocess fermentation media using direct injection is presented. The
validation studies show a satisfactory selectivity, linearity, accuracy, and
recovery of the method. The lowest concentration detectable for glucose and
methanol is 3.5 and 6.7 mg/100 mL, respectively. This method could be an
attractive choice for the analysis of these compounds not only in fermentation
media but also in biomedical and environmental samples.
PMID: 10890750 [PubMed - indexed for MEDLINE]
PUBMED Search: Glycero AND GC-MS AND (method OR analysis)
1: J Chromatogr B Biomed Sci Appl 2000 Jul 7;744(1):47-54
Determination of stable isotopic enrichment and concentration of glycerol in
plasma via gas chromatography-mass spectrometry for the estimation of lipolysis
in vivo.
Flakoll PJ, Zheng M, Vaughan S, Borel MJ.
Department of Surgery, Vanderbilt University Medical Center, Nashville, TN
37232, USA. paul.flakoll@mcmail.vanderbilt.edu
Measuring glycerol's rate of appearance into the plasma compartment provides an
excellent estimation of whole-body lipolysis. The glycerol rate of appearance
can be calculated by estimating the plasma dilution of continuously infused
stable or radioactive isotopes of glycerol. Previously, determination of
glycerol stable isotopic enrichment has required either chemical ionization gas
chromatography-mass spectrometry (GC-MS) or electron impact ionization GC-MS in
which a fragment containing only a portion of the glycerol molecule was
measured. The present method uses tert.-butyldimethylsilyl (tBDMS)
derivatization and electron impact ionization to measure a fragment including
the entire glycerol molecule. The method determines concentration and enrichment
of plasma glycerol in a simple, precise, and cost-efficient manner, providing a
basis from which lipid homeostasis can be assessed.
PMID: 10985565 [PubMed - indexed for MEDLINE]
2: Anal Biochem 1992 Aug 15;205(1):172-8
A mass spectrometric method for measuring glycerol levels and enrichments in
plasma using 13C and 2H stable isotopic tracers.
Gilker CD, Pesola GR, Matthews DE.
Department of Medicine, Cornell University Medical College, New York, New York
10021.
The stable isotope tracer [1,1,2,3,3,-2H5]glycerol has been commonly used as a
tracer to measure glycerol kinetics and lipolysis in vivo. The method for
measuring samples using the trimethylsilyl derivative and electron impact gas
chromatograph-mass spectrometry retains only three of the five deuteriums,
resulting in the possibility of incorrectly identifying the whole glycerol
tracer molecule. This reports preparation of glycerol as the heptafluorobutyrl
derivative and measurement by negative ion chemical ionization gas
chromatography-mass spectrometry to produce a derivative with an intense
molecular ion that retains all five deuterium labels. Thus the heptafluorobutyrl
derivative analyzed by negative ion mass spectrometry overcomes the problems
associated with fragmentation and loss of the isotopic label. Glycerol
concentration using a labeled internal standard can be determined in plasma with
a precision of 3%. Nanomole amounts of glycerol can be analyzed for 13C or 2H
enrichments with a precision of +/- 0.14 mol% excess isotope. This simple,
sensitive method for measuring glycerol levels and stable isotopic enrichment in
plasma uses a simple extraction procedure and requires a minimal volume of
plasma (less than 300 microliters).
PMID: 1443555 [PubMed - indexed for MEDLINE]
3: J Chromatogr 1992 Jul 1;578(1):1-7
Determination of "free" glycerol in human serum reference materials by
isotope-dilution gas chromatography-mass spectrometry.
Bernert JT Jr, Bell CJ, McGuffey JE, Waymack PP.
Division of Environmental Health Laboratory Sciences, Centers for Disease
Control, Atlanta, GA 30333.
Serum free glycerol analyses are an important part of the preparation and
evaluation of human serum reference materials used for the quality assurance of
triglyceride assays. However, enzymatic kits for free glycerol analysis obtained
from different vendors have, on occasion, provided different results for a given
sample. In an effort to establish the "target" glycerol content of selected
reference materials, we have established a method for the analysis of serum free
glycerol by using isotope-dilution gas chromatography-mass spectrometry,
incorporating [1,3-13C2]glycerol as the internal standard. The use of a
simplified serum extraction and clean-up procedure resulted in (uncorrected)
recoveries of glycerol averaging about 90% before derivatization, and in
estimated concentrations for spiked serum pools that corresponded closely to the
expected values. A comparison of enzymatic and gas chromatographic-mass
spectrometric results for several reference serum pools suggest that the latter
method is of value in evaluating and validating routine enzymatic methods for
free glycerol analysis.
PMID: 1400773 [PubMed - indexed for MEDLINE] |
