I thought I would write my question over to inform you of the
information that I didn't give you. I am trying to purify a protein
and I want to use FRACTIONATION as one of the steps in the process.
After the protein is broken up, it is given over to fractionation for
further purification. If this is not enough information please let me
know.

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Request for Question Clarification by librariankt-ga on 28 Sep 2004 07:09 PDT

Ted -
There are a couple of different ways of purifying proteins by
fractionation (which is the process of dividing things until you get
just the bit you really want) - the most common is fractionation using
a centrifuge.  Do you know if that is the process you have access to,
and if so, do you need a procedure for doing it?
- LKT

You can fractionate proteins based on any of their characteristics.
Comonly used criteria are size, pI, charge, hydrophobicity and S
value. Techniques include centrifugation, electrophoresis,
chromatography, solubility, immunology...  In order to know how pure
your sample is you will need some way of identifying the protein of
interest as well as the total ampount of protein at each step.