My hypothesis is to purify a UNKNOWN PROTEIN but fisrt i want to
purify a known protein so that i can know how to do one that is
already known. This is the answer to the question you asked. I don't
know the name of the protein i am seeking, i just want want to get the
techniques down. Will you please help.
                                                                           Ted.

Go to your local library and pick up a biochemistry text book.  I
would recommend Garrett and Grisham, Biochemistry 3rd Edition. Check
out this too, it might be useful, Shawn Farrell's Experiments in
Biochemistry.  There are a LOT of techniques for protein purification.
 Far too many, and far too complicated to explain here.

Ted, try clicking the "Clarify question" button on a question you've
already asked instead of opening a new question every time you want to
add something.  Questions are usually read separately, without a
context, and not as a dialogue.  If you want to respond to a
researcher who asks you a clarifying question, stay on the same page
and just clarify it.  Your questions have been very hard to decipher
and could use some clarification.

Also note that researchers' names appear in blue and underlined
because they are links.  Others, like me, are just commenters and not
the paid researchers who supply answers in this service.

Just trying to be helpful--
Archae0pteryx
(not a researcher)