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Q: Cytochrome C ( Answered,   2 Comments )
Subject: Cytochrome C
Category: Science > Biology
Asked by: tedmccall-ga
List Price: $40.00
Posted: 09 Nov 2004 10:25 PST
Expires: 09 Dec 2004 10:25 PST
Question ID: 426692
Want to know the steps or techniques in purifying CYTOCHROME C. I just
want to know the steps or techniques, in this process. I am trying to
be precise. Since i am trying to find a UNKNOWN PROTEIN, i would like
to know the steps of this protein rather then buy one that has already
been put together. I want to learn the techniques and steps of this
protein.          Ted.
Subject: Re: Cytochrome C
Answered By: jdb-ga on 29 Nov 2004 10:16 PST
Hello, I am following up on your question about steps needed for the
purification of Cytochrome C. Here are some sites that describe the
process in detail.

Cytochrome c purification

Clarification of Answer by jdb-ga on 29 Nov 2004 10:34 PST
Hello again,

Apologies, I inadvertently hit the "Post" button before I finished
pasting in the information. Here is what I have found.

Smith University - Biochemistry
Purification Protocol 
Results Table 
Column Preparation 
Gel Filtration 
Ion Exchange 
Protein Determination 
Cytochrome Determination 

The full-text articles below include descriptions of processes used to
purify cytochrome c.

Biochem. J. (2000) 349 (153?158)
"Identification and characterization of a novel cytochrome c3 from
Shewanella frigidimarina that is involved in Fe(III) respiration"
Euan H. J. GORDON*1, Andrew D. PIKE?, Anne E. HILL*, Pauline M.
CUTHBERTSON*, Stephen K. CHAPMAN? and Graeme A. REID*2
*Institute of Cell and Molecular Biology, University of Edinburgh,
Mayfield Road, Edinburgh EH9 3JR, U.K., and ?Department of Chemistry,
University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, Scotland,

Appl Environ Microbiol. 2003 August; 69(8): 4727?4731.
doi: 10.1128/AEM.69.8.4727-4731.2003. 
"A Cytochrome c from a Lupanine-Transforming Pseudomonas putida Strain
Is Expressed in Escherichia coli during Aerobic Cultivation and
Efficiently Exported and Assembled in the Periplasm"
Mustak A. Kaderbhai,* David J. Hopper, Kalim M. Akhtar, Syed K.
Abbas,? and Naheed N. Kaderbhai
Institute of Biological Sciences, University of Wales, Aberystwyth
SY23 3DD, Wales, United Kingdom

I hope these are useful. Please let me know if I can be of further
assistance.  jdb-ga
Subject: Re: Cytochrome C
From: mikewa-ga on 10 Nov 2004 08:04 PST
Come on!! All you have to do is type ' "cytochrome c" purification '
into Google and you get your answer. Why would you be willing to spend
$40 for this?
Subject: Re: Cytochrome C
From: robd1752-ga on 29 Nov 2004 08:47 PST
I hope you understand that there is no one way to purify a protein.
There are dozens of different protein purification techniques, and
most methods use a combination of at least two of these, with the
conditions uniquely tailored. Knowing how to purify CytC will not help
you to purify anything else.

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