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Subject:
Microbiology
Category: Science > Biology Asked by: geertha-ga List Price: $2.00 |
Posted:
14 Feb 2006 10:06 PST
Expires: 16 Mar 2006 10:06 PST Question ID: 445705 |
How to control bacterial contamination? |
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There is no answer at this time. |
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Subject:
Re: Microbiology
From: karizma-ga on 15 Feb 2006 02:57 PST |
In what? Food is treated radically different from eg. surgical instruments. Please specify a bit more. |
Subject:
Aseptic technique
From: eddyinsf-ga on 25 Feb 2006 20:59 PST |
Here's a simple answer to your open-ended question: Bacterial contamination is typically controlled by using an autoclave (uses wet head), irradiation (bombardment with subatomic particles damages molecules), uv light (causes dna damage), antibiotics (most were discovered from molds), chemicals (e.g. ethanol, chlorine), refrigeration (at <= 5 Celcius growth is practically halted), or by controlling nutrient/gas requirements (e.g. lack of oxygen would kill obligate aerobes). Which method depends on the application/purpose. For example, are you trying to sterilize your growth media? Are you trying to selectively favor the growth of one organism and supress the growth of others? Are you trying to preserve food from spoiling? Are you trying to avoid infection during an operation? Aseptic technique is a set of practices used by biologists to limit contamination, both of the biologist him/herself and the sample(s) being handled. It involves, for example, wiping down surfaces with 70% ethanol (10% bleach works too), working under a laminar flow hood, avoiding any contact with skin (e.g. wear gloves), etc. Good luck, Ed the biologist |
Subject:
Re: Microbiology
From: shakakai-ga on 27 Feb 2006 22:27 PST |
Everything that Ed stated above is correct but I would also like to add that you can use what's known as a flow hood to keep out bacterial spores that may contaminate your experiment. Flow hoods pump steriled air over the experiment table keeping any airborne particulates away from your testing substance. Away from the hood you have to make sure that the experiment container is sealed. Combining this technique with the ones Ed listed above will normally do the trick unless you are using particularly susceptible growth medias. Cheers, Todd the Geneticist |
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