To Andrewxmp. OK,i will tell you the whole story. I want to purify a
UNKNOWN PROTEIN from the yeast cell. But before i can do this, i will
have to purify a KNOWN protien. All the information that i gain from
this purification on the KNOWN will be used on the UNKNOWN PROTEIN.
You said to specific, well i have decided to purify the CYTOCHROME C
first. I am trying to purify Cytochrome C protein first for practice.
This is about as much detail that i can go. I don't know what you mean
by "extract". If i need to be more specific than this, i will try.
Please help me to get on the right track. Try to bring some logic to
all of this.                     Ted.

To respond to Andrewxmp, you may want to go back to your earlier
question, rather than starting a new question:

http://www.answers.google.com/answers/threadview?id=457392

find a collage level chemistery book, the vast majority contain
experimental procedures on a whole range of compounds