I would like to have a COMMENT about how to get started purifying a
protein such as Cytochrome C. I just want to a few tips on how to do
this.

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Request for Question Clarification by pafalafa-ga on 02 Feb 2005 13:15 PST

This paper describes the methods for purifying cytochrome c:


Pollock, W.B., Rosell, F.I., Twitchett, M.B., Dumont, M.E., and Mauk,
A.G. 1998. Bacterial expression of a mitochondrial cytochrome c.
Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline
conformational transition. Biochemistry 37: 6124?6131


A good librarian should be able to help you obtain a copy of the paper.  

Best of luck,

pafalafa-ga

First, you turn off your internet service, and go to the books upon
your shelf, and read.

Next, you CHOOSE...

Then, you TRY...

Then.... you try try try again!

You're armed with a wealth of stuff already Ted, go forth! be
confident!  make a mess!  Have fun!  You can do it grasshopper!

Safety first.

I suggest you e-mail someone who has already purified Cytochrome C in
a published article that you find interesting. Tell them briefly about
your project (just a few words) and ask them for a protocol and some
hints.

Good luck!

It seems to me that it's a maybe little improper and definitely
impolite to post a google question, but specify that you just want
someone to answer your question by leaving a comment.

If you want to post a question, but have no intention of paying should
your question be answered, there's a place for you, and it's called
usenet.

http://groups.google.com is the place to start. However, don't expect
that they will say anything different from what the experts here say,
which I will repeat.
It's such a big question, you need to consult the many good volumes
available at your college's library on the subject.

get your hands on any collage level chemistry book (tome) and they
usually have purification methods in it!!!!