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Q: science - receptor modification ( No Answer,   1 Comment )
Question  
Subject: science - receptor modification
Category: Miscellaneous
Asked by: val1-ga
List Price: $10.00
Posted: 04 Apr 2005 09:54 PDT
Expires: 04 May 2005 09:54 PDT
Question ID: 504740
What is a simple way to modify a protein and add a nuclear localizing
sequence so it can translocate to the nucleus?
Answer  
There is no answer at this time.

Comments  
Subject: Re: science - receptor modification
From: xarqi-ga on 04 Apr 2005 17:17 PDT
 
The coolest way (IMHO) is to put part of the antennapedia sequence at
the beginning of the protein.  Just dump the recombinant protein into
the medium, and it'll end up in the nucleus!

(This is just from memory, so a bit of searching might get you
something more specific in terms of a method, or prove me totally
wrong!)

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