What is the nucleotide sequence of the RBG4 plasmid?  I have a
restriction map that says that it's 3812 base pairs long, but I have
not been able to find the full sequence online.  RBG4 may also be
refered to as pRBG4 in the literature.

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Clarification of Question by armard-ga on 19 Apr 2005 10:52 PDT

Yes, I'm sure I dont want the prpg gene.  I have HA and FLAG tagged
GluR1 flip in RBG4, but I got them from a lab who hadn't used them in
years, and had lost all documentation.

I couldn't find it either.
I assume you have Lee et al. (1991) J Biol Chem 266(18) 11448.


Options include:
Emailing the corresponding author of that paper;
Deriving the sequence from the expression vector construction procedure given.

we have that in my biochemistry module, but i can't find the sequence
anywhere either, i don't think we're aloud to know!!
(it's the password for the noble prize winners club he he!!)

Are you sure that you don't want the prgp gene, which is part of the
pCF10 plasmid? If so, here's the link:

http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=58616081&itemID=72&view=gbwithparts

May I ask why you need the sequence information?  Perhaps there is
another way to do what you want done.