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Subject:
standard curve
Category: Science > Biology Asked by: microbiol-ga List Price: $10.00 |
Posted:
28 May 2005 14:13 PDT
Expires: 27 Jun 2005 14:13 PDT Question ID: 526709 |
If you generate a graph with bacterial concentration (cfu/ml) vs turbidity units, could you use this same graph in future experiments? And when would you need to generate a new curve? What's the difference between the graphs: turbidity (OD660) vs time and cfu/ml vs time? |
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There is no answer at this time. |
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Subject:
Re: standard curve
From: andrewxmp-ga on 30 May 2005 22:44 PDT |
There is no difference, the two graphs should be perfectly proportional because the higher optical density is DUE TO refraction of light by the bacteria, so there will be a direct correlation. You theoretically could use this curve again, however people generally dont because slight factors (temperature, small errors in measuring, etc) can affect it. Usually, one creates a standard curve with the mixture that will be measured, at the same time both the standards and the experimental mixtures, and a new curve is made for each experiment. If you would like me to elaborate on this and get some sources to better explain the procedure, I will gladly do so and post it as an answer. Please let me know if this would be useful. Thanks! Andrewxmp |
Subject:
Re: standard curve
From: mikewa-ga on 01 Jun 2005 04:54 PDT |
There are acouple of factors that could lead to errors. The turbidity is dependent on cell size, as well as numbers and the cfu value assumes no clumping of cells and 100% viability. Since all three may vary from culture to culture you cannot assume the relationship will always be the same. However: for the same strain, grown under the same conditions and for the same length of time, the results from one test will be a pretty close match to the next. |
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