Hello tedmccall-ga,
I?ve found a selection of books that should help you with basic
instructions for doing lab work in protein purification. I?ve also
found some online resources that offer collections of lab protocols
for protein purification prepared by experts in the field that might
be useful to you.
Best wishes for your further studies.
~ czh ~
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PROTEIN PURIFICATION LAB PROTOCOLS -- BOOKS
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http://www.amazon.com/exec/obidos/ASIN/0130100749/gemotrack12-20/ref%3Dnosim/102-1396315-9348923
Microbiology Laboratory Fundamentals and Applications (2nd Edition)
by George A. Wistreich
Editorial Reviews
From Book News, Inc.
Consists of 70 experiments that demonstrate how to apply the concepts,
principles, and techniques used in microbiology. The laboratory manual
covers cultivation and staining techniques, the metabolic activities
of bacteria, sterilization and disinfection, fermentation, immunology,
epidemiology, and medical microbiological. The second edition adds new
illustrations and procedures. Book News, Inc.®, Portland, OR
Book Description
This comprehensive laboratory manual provides state-of-the-art
techniques, concepts, and applications of microbiology. The overall
approach is designed to start with basic concepts and procedures and
to gradually build more advanced levels, strengthening readers'
understanding and skills through the process. An invaluable reference
as a refresher of basic concepts, techniques and procedures. Current,
thorough coverage of topics including, but not limited to: cultivation
techniques; microbial genetics; immunology; and medical microbiology.
For laboratory technicians and medical laboratory technologists.
***** This is an excellent book for learning lab fundamentals. You can
then take what you?ve learned and apply it to your specific tasks in
protein purification.
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http://www.amazon.com/exec/obidos/tg/detail/-/081763665X/ref=pd_sim_b_6/102-1396315-9348923?%5Fencoding=UTF8&v=glance
Protein Analysis and Purification: Benchtop Techniques
by Ian M. Rosenberg
Editorial Reviews
Book Description
This comprehensive, practical and user-friendly manual engages the
novice and the veteran alike in the cutting edge of protein research.
It is designed to be a practical progression of experimental protocols
that an inexperienced investigator may follow when embarking on a
biochemical or biotechnology project. The manual will also appeal to
experienced researchers and graduate students as a benchtop handbook
which, without sacrificing scientific concepts, maintains the spirit
of experimentation. The book guides the reader through a plethora of
proven and productive methods for both soluble proteins and membrane
or particulate-associated proteins, including protein quantitation,
chromatography, electrophoresis, immunochemical methods, protein
labeling, peptide mapping, protein localization, protein modification
analysis, recombinant protein expression and more. Special attention
has been given to an optimal choice from a rich variety of methods
available in a modern lab for a particular application.
Features: * More than 180 protocols are included with materials lists,
step-by-step instructions and valuable comments on practical issues
such as time considerations and safety issues. The protocols may be
performed in the order laid out, or may be used independently. The
first option is best for a novice or for student instruction, the
second one is fit for experienced researchers.
* Each chapter contains a wealth of practical information, including
troubleshooting tips, safety icons and references. The comprehensive
appendices present conveniently in one place frequently used tables,
conversion charts, equipment guidelines and vendor addresses.
This manual is a must for all laboratory libraries and an invaluable
tool at the bench, filling a basic and vital need of workers in all
fields of life sciences research. Selected chapters and protocols will
serve as lab courses in contemporary protein research techniques.
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http://www.amazon.com/exec/obidos/tg/detail/-/0199636737/qid=1120616136/sr=8-1/ref=pd_csp_1/102-1396315-9348923?v=glance&s=books&n=507846
Protein Purification Techniques: A Practical Approach (Practical Approach Series)
by Simon Roe (Editor)
Editorial Reviews
From Book News, Inc.
It's been ten years since publication of the first edition of this
text. The second edition provides the most recent strategies and
detailed practical procedures for laboratory-scale protein
purification from common sources. Coverage includes fusion methods;
initial purification of inclusion bodies; purification of
crystallography; and purification from mammalian cell culture,
microbial cell culture, milk, animal tissue, and plant
sources.Copyright © 2004 Book News, Inc., Portland, OR
Book Info
Describes strategies and practical procedures for protein purification
from common sources and includes chapters on the purification of
inclusion bodies, fusion proteins, and proteins for crystallography.
Previous edition was titled, Protein Purification Methods, c1989.
Softcover, hardcover also available.
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http://www.amazon.com/exec/obidos/tg/detail/-/0122135857/ref=pd_sim_b_2/102-1396315-9348923?%5Fencoding=UTF8&v=glance
Guide to Protein Purification : Volume 182: Guide to Protein
Purification (Methods in Enzymology Series, Vol 182)
by John N. Abelson, Melvin I. Simon, Murray P. Deutscher
***** Be sure to check the ?check inside? feature for this book as
well as the Customer Comments.
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http://www.sigmaaldrich.com/Area_of_Interest/Equipment_Supplies__Books/Books/Proteomics.html
PROTEOMICS & PROTEIN EXPRESSION
Accelerating Proteomics Research Through Integrated Solutions
***** This site offers a large selection of books that might be of interest to you.
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PROTEIN PURIFICATION LAB PROTOCOLS ? ONLINE RESOURCES
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http://www.bio.com/aboutbio/index.jhtml;jsessionid=P3JPUIDIZL0T1R3FQLMSFEWHUWBNQIV0
bioProtocol
About Protocols
-- Protocols contributed by scientists from over 125 academic laboratories
-- World renowned Editorial Board
-- Wide variety of life science disciplines covered
-- Searchable protocol database
-- Printable versions available for all protocols
-- Store protocols of particular interest to you and send protocols
to your colleagues using the My Protocols feature
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http://www.bio.com/protocolstools/methodology.jhtml?id=psc8K
Discipline à Yeast à Methodology à Isolation and Purification of Proteins
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http://www.protocol-online.org/info.html
Protocol Online is a database of research protocols in a variety of
life science fields. It contains protocols contributed by worldwide
researchers as well as links to web protocols hosted by worldwide
research labs, biotech companies, personal web sites. The data is
stored in a MySql relational database. Protocol Online also hosts
discipline specific discussion forums (BioForum), and provides a free
PubMed search and alerting service (PubAlert). This site was created
in June 1999 and is maintained by Dr. Long-Cheng Li. In order to
better serve the research community, any comments and suggestions that
can help improve this site are welcomed.
http://www.protocol-online.org/prot/Molecular_Biology/Protein/Extraction___Purification/
Top à Molecular Biology à Protein à Extraction & Purification
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http://ihcworld.com/
IHC World, dedicated to everything immunohistochemistry, provides the
most comprehensive and up-to-date information about
immunohistochemistry. The Protocol Database is compiled based on
published literatures, laboratory submissions, as well as individual
experimental data. The Discussion Forum is a great resource to ask and
answer questions, discuss and share protocols and tips. The Image
Gallery is to enhance communication between researchers in
immunohistochemistry community. Users can upload images, discuss and
share comments on images of interest. IHC Mall, the buyer's guide for
histology professionals, gives you direct access to histology and
immunohistochemistry products and services offered by suppliers
worldwide.
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http://ihcworld.com/neuroscience/protein_chemistry.htm
Protein Chemistry Methods
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