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Subject:
Cell culture lab setup
Category: Science > Biology Asked by: djjc-ga List Price: $2.00 |
Posted:
29 Jun 2005 11:45 PDT
Expires: 15 Jul 2005 10:28 PDT Question ID: 538401 |
I would like to sepup a cell culture laboratory. I would like to know basic information on what kind of equipments needed, where to purchase, space needed, cost etc. |
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There is no answer at this time. |
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Subject:
Re: Cell culture lab setup
From: mikewa-ga on 29 Jun 2005 12:58 PDT |
Do you have, or have access to standard lab equipment? i.e. autoclave, centrifuges, -80 freezer etc. |
Subject:
Re: Cell culture lab setup
From: djjc-ga on 29 Jun 2005 15:42 PDT |
not at present time. Thanks. |
Subject:
Re: Cell culture lab setup
From: pforcelli-ga on 29 Jun 2005 20:15 PDT |
Centrifuge Vortexer hot plate thermometer fume hood culture incubator uv lamp water purifier (if you aren't in a lab facility that supplies deionized water) microscope Fridge, Freezer (-4, -80) micropipetters (P10, 20, 100, 1000) pH meter microgram balance lab oven autoclave electrophoresis power supply and sundry accessories cryogenic equiptment alcohol burner (I'm assuming you don't have access to gas) and of course no lab could be complete without a waring blender. So I'd say that probably covers the basics in terms of general lab equiptment. On to cell culture specific... Culture media Coated plates, dishes, flasks inoculating loops, needles, spreaders slides cell tubes plus your basic lab chemical needs and glassware needs. if you check out sigmaaldrich.com, they have a good set of product descriptors for lab start up. Same with fisherscientific. I'm not going to even try to give a detailed breakdown of the above, but daigger sells all of the equiptment. I'd estimate 10k-15k in terms of start up cost. In terms of space, that you don't need to much of, so long as you can move around and have an area dedicated to your lab and only your lab, I'd say a 100sq ft room would do the trick. As I said, thats my quick and dirty estimate for you. Lab startup isn't easy or cheap. |
Subject:
Re: Cell culture lab setup
From: mikewa-ga on 30 Jun 2005 04:40 PDT |
I would put the price tage MUCH higher: at least $100K |
Subject:
Re: Cell culture lab setup
From: dops-ga on 05 Jul 2005 13:21 PDT |
Once again I'm going to have to agree with mikewa. The cost of the -80 alone could put you at 10,000 add the other equipment (30K for the autoclave, 1-10K for the centrifuge...) In addition to pforcelli's list you'll most certainly need a laminar flow hood (not a minor expense). When we set up our facility we met with some very helpful representatives from VWR, who were more than willing to compile a complete list. Perhap you could contact them. |
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