Would like to know a common name of a experiment that has to do with
purifying a protein. Just the name of a experiment that have to be
done. I would like to know its name.

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Request for Question Clarification by pafalafa-ga on 15 Aug 2005 12:26 PDT

Hello Ted.

Here's a common college-level laboratory experiment regarding protein purification:


http://www.as.ysu.edu/~tdkim/CHEM785/a-Lactablumi.PDF
Experiment 3: Purification and Characterization of a-Lactalbumin, a Milk Protein


Is this the sort of experiment you had in mind?


pafalafa-ga

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Request for Question Clarification by pafalafa-ga on 16 Aug 2005 07:00 PDT

Ted,

I saw the other question that you posted, asking for more information.  

Rather than explain the document I linked to earlier, I thought it
might be even more helpful for you to have a look at this manual on
protein purification:


http://www.biochem.uiowa.edu/donelson/Database%20items/protein_purification_handbook.pdf


It covers the major steps (protein capture, protein isolation, and
polishing) and the techniques used.


Have a look, and then let me know what additional information you still need.

Cheers,

paf

AFFINITY CHROMATOGRAPHY
is the name of the most used method in the lab.

But usually  the purification is a two-step process:

1) 
   PROTEIN PRECIPITATION eliminate what is not a protein.
   You could stop here if you want a pool of proteins.


   
2) AFFINITY CHROMATOGRAPHY is based on the features of the specific      
protein that you want,so you can catch what you want between a group    
of other proteins.



pic for affinity chromatography:
http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1/ch5_affinity.jpg


info on some methods:
http://www.biotech.vt.edu/classes/bion_4784/9-ProteinPurification/ProteinPurification.html