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Subject:
How to test percentage of crude protein?
Category: Science > Agriculture and Farming Asked by: josephltt-ga List Price: $10.00 |
Posted:
18 Oct 2005 05:08 PDT
Expires: 17 Nov 2005 04:08 PST Question ID: 581622 |
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There is no answer at this time. |
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Subject:
Re: How to test percentage of crude protein?
From: hfshaw-ga on 21 Oct 2005 14:35 PDT |
It would help to know the nature of the material you want to test. Is it a solution? Solid? Suspension (e.g., blood)? What's the nature of the non-protein material in the sample? |
Subject:
Re: How to test percentage of crude protein?
From: ike9898-ga on 22 Nov 2005 11:33 PST |
Crude protein is normally determined by measuring the amount of nitrogen in a sample and then using a conversion factor to estimate how much protein that represents. The classic mehtod is the Kjeldahl Nitrogen assay. This method is well documented and known by many food chemists. It is relatively slow and labor intensive. If you can make a much bigger investment, there are automated systems that determine the concentration of nitrogen. We use one from Leco Corp. Both these methods would be laboratory based and not particularly cheap if you don't already have the equipment. As far as something cheap and portable, I think you're going to have a hard time. If you can be sure that all your protein is soluble, you can use one of many commercially available kits for soluble protein concentration. Most of these depend on the use of a spectrophotometer or a fluorimeter. It might be possible to use something like the Bicinchoninic Acid Assay, and visually compare the color of the solution to a standardized color chart. This would only give you a very rough idea of protein concentration. |
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