Category: Science > Biology
Asked by: natsaratsa-ga
List Price: $5.00
23 Apr 2006 18:02 PDT
Expires: 26 Apr 2006 19:11 PDT
Question ID: 722098
Why did the use of DNA polymerase from the bacterium THermus aquaticus allow researchers to add the necessary reagents to tubes in a preprogrammed heating block (PCR)?
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Re: bacterial DNA
From: pforcelli-ga on 23 Apr 2006 18:37 PDT
Hey, Great Question! The Taq polymerase is heat resistant, which is key, as it can be raised to a temperature beyond its functional range to allow for the melting of the primer and copy strands in the chain reaction. This is repeated through many cycles, each time exponentially increasing the number of copies of the target sequence. Taq Pol made this possible because it isn't denatured at temperatures which melt dsDNA.
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