Category: Science > Biology
Asked by: natsaratsa-ga
List Price: $4.00
15 May 2006 06:51 PDT
Expires: 14 Jun 2006 06:51 PDT
Question ID: 728956
If you were using a bacterial culture to grow and count viruses, and you ended up with no plaques on your plate, what might an explanation be?
|There is no answer at this time.|
Re: culturing viruses
From: akt123-ga on 23 May 2006 23:09 PDT
Acually there are two type of bacteriophage (virus surviving and replicating in bacterial host). First type include LYTIC PHAGES: these are the virus which cause the lysis or death of cell and released in outside environment. In these kind, phages DNA or RNA replication is immediately followed by synthesis of capsid proteins and these are assembled togethor resulting in the formation of complete phage particle which is released after causing lysis of bacterial cell. Second types of phages are LYSOGENIC phage: these are the phages which can integrate the genetic material (DNA) with the host bacterial DNA and replicate generation after generation in this form only. When the bacterial cell divide phage genetic material can also be transferred to daughter cells in integrated form. This can continue for several generations without any harm (lysis) to bacterial cell. One example of these kind of phages is Lambda phage. Thus i feel now you can understand why sometimes there is no plaque formation even after phage infection. But always remember that lysogenic phages switch to lytic mode at sometime during future generation and lyse the cell. There is one more category of phage which don`t integrate in host cell DNA but multiply independently in host cell. These are released in outside environment without lysis of cell (most probably by budding) and infected host cell can survive. ex. M13 phage
If you feel that you have found inappropriate content, please let us know by emailing us at email@example.com with the question ID listed above. Thank you.
|Search Google Answers for|