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Q: growing chlorella at home ( No Answer,   4 Comments )
Question  
Subject: growing chlorella at home
Category: Miscellaneous
Asked by: timespacette-ga
List Price: $5.00
Posted: 28 May 2006 21:37 PDT
Expires: 27 Jun 2006 21:37 PDT
Question ID: 733225
Can someone find some online resources that explain how to grow your own chlorella?

Chlorella:  a single-celled, blue-green algae that grows in fresh
water.  Used widely as a nutritional supplement.


***
Answer  
There is no answer at this time.

Comments  
Subject: Re: growing chlorella at home
From: probonopublico-ga on 30 May 2006 23:42 PDT
 
Congratulations, Timely One

You have defeated all 500 Researchers (including Pinkfreud-ga,
Rainbow-ga and Politicalguru-ga) as well as all those extraordinary
Commenters like Bowler-ga, Myoarin-ga, Frde-ga and (unbelievably)
Kemlo-ga.

Me, too, I am afraid but from what I have been able to find, the stuff
just GROWS without any help from humans whatsoever, provided it's got
some water in which to do its stuff.

And, from what I know, water is something that you've got in spades.

So, I reckon that all you've got to do is get a supply of the stuff
and chuck it in the water and then Watch Out!

Oh and should you be thinking of inviting me round for a meal, please
don't be offended if take mine home in a doggy bag.

(As you may know, Daisy will eat ANYTHING.)

I hope that my Comment exceeds your expectations (a la Tutuzdad-ga)
but before giving me the obligatory FIVE STARS, please also give a
very generous tip. (I'm sure that you can afford it after all the help
that was provided for your safe blowing project.)

Looking forward (a la Denco-ga)

All the Best 

Bryan
Subject: Re: growing chlorella at home
From: frde-ga on 31 May 2006 03:00 PDT
 
http://sti.srs.gov/fulltext/ms2000638/ms2000638.html

|Algal cultures included Chlorella pyrenoidosa, Scenedesmus
quadricauda, Closterium sp. and Cyanidium caldarum. All four strains
were obtained from the Carolina Biological Supply Company, Burlington,
NC. Chlorella and Scenedesmus were grown in modified Bold Basal (BB)
medium (Nichols and Bold 1965). Closterium was grown in Alga Gro
Freshwater Medium and Cyanidium in Doemel's Cyanidium medium (Carolina
Biological Supply Co., 1978.)

Culture media was prepared in 25 mL batches in 50 mL flasks prior to
being autoclave sterilized. Flasks were subsequently inoculated with
600 m L of algal culture. The flasks were then placed in a Pschrotherm
shaker/incubator (New Brunswick Scientific) for two weeks at 20°C,
with a 12 h light (200 m E-2 s-1):12 h dark illumination regime and
100 rpm rotation. Control flasks, not inoculated with algae but
otherwise treated identically with all treatment conditions, were
examined for nitrate and gadolinium concentrations at the start and
conclusion of all three experiments.|
 
So modified Bold Basal, light and dark, 20°C and a good shake come into it.

Somewhere else I found that they were bubbling CO2 through it,
unfortunately my browser packed up on that page.

I'll post things before the browser dies completely
Subject: Re: growing chlorella at home
From: bowler-ga on 31 May 2006 10:58 PDT
 
Is this helpful?

"... Chlorella is the algae we are growing in our photosynthetic bioreactor..."

http://www.cascadehs.csd.k12.id.us/advbio/97-98/minicoil.html
Subject: Re: growing chlorella at home
From: sparkysko-ga on 07 Aug 2006 13:48 PDT
 
Hello!

So it's algae ye be wanting eh?

Algae is a plant, like any other plant, it needs a few things to grow.

1) Light
2) Nutrients
3) Water
4) Co2 and Oxygen
5) A Starter

1) Lighting. You're going to need ALOT of lighting. Like. ALOT. A good
rule of thumb is here at least 5 watts of compact fluorescent lighting
per gallon of water (Minimum of 50 watts). I'd recommend as much as
you can put on it. You're trying to simulate sunlight here. I like
those screw in fluorescent bulbs. The largest size possible. They're
cheap, and put out a ton of light. You'll want to use many of them.

2) Nutrients. Algae needs at the least phosphates. Ideally a good mix
of everything. Hydroponic fertilizer is great, but if that's not
available, try some miracle grow in a dropper bottle, or crush up
miracle grow tablets.

3) Water - Ideally dechlorinated. Lake or river water is best if
available since it's already 'matured'. Use bottled water, or tap as a
last resort. Bottled before tap. (No chlorine).

4) CO2 naturally diffuses into the water from the water surface. Same
with oxygen. You don't want this in a completely sealed container, or
else it might die off (PH will change from CO2, etc). Some speculate
that algae can use additional Co2 to turbocharge it, while others
disagree. You can brew CO2 in a 2 liter bottle with sugar and yeast,
and run a pipe from the cap in the bottle into your algae culture.
This will bubble Co2 through it. Normal plants *DO* benefit from Co2,
but Algae might not. Lighting needs to be adequate before this will
benefit it.

Oxygen - If you're lacking oxygen, the nutrients will invite fungus,
and you will be growing nasties instead of green algae. Too many
nutrients will cause this. Also poor oxygen exchange with the surface.

5) Just leaving your container out will get algae spores. If you want
to grow a specific type, you can try to buy a starter, or collect your
own algae source. However it might be out-competed with other types of
algae. The only way to prevent this is in a sterilized lab environment
where you can ensure that there is nothing but the algae you want (not
easy to do, needs to be done in a clean room environment where no
spores are, good luck).

At the simplest, what you could try is a 2 liter bottle with some
fertilizer. Maybe throw some dirt in there. Put this outside, or in
bright light. I have found that leaving containers outside can also
kill them off when the temperatures gets too extreme. Lots of
nutrients, and patience is key.

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