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Q: Milk Spoilage Detection ( Answered,   2 Comments )
Question  
Subject: Milk Spoilage Detection
Category: Science > Agriculture and Farming
Asked by: impostervt-ga
List Price: $12.00
Posted: 16 Jan 2003 19:08 PST
Expires: 15 Feb 2003 19:08 PST
Question ID: 144545
I am interested in knowing about how to scientifically detect spoilage
of milk in a non-labratory setting. Specifically, what bacteria causes
pasturized, packaged milk to spoil (the type you buy from a grocery
store) and what are fast ways to detect them?  I do not need to know
which type of bacteria caused the spoilage, just the ability to
recognize it has spoiled.  Particularly helpful would be to know if
any commercial products exist which are used on milk cartons
themselves to show if the milk in the package has spoiled.
Answer  
Subject: Re: Milk Spoilage Detection
Answered By: tutuzdad-ga on 18 Jan 2003 16:15 PST
 
Dear impostervt-ga;

Thank you for allowing me an opportunity to answer your interesting
question.

Let’s see; you want to know how to “scientifically detect spoilage of
milk in a non-laboratory setting”. Ok, at the risk of sounding
facetious (and I assure you that is not my intent) I must get this out
into the open before we proceed:

The best way to tell if your milk is spoiled is to check the SELL BY
date or EXPIRATION date on the container. If it is more than six days
beyond this stated date, you can safely assume that the milk is
spoiled or well on its way to being spoiled. CSIRO (CSIRO is
Australia's Commonwealth Scientific and Industrial Research
Organisation) and the “Austrailian Food Industry Science Centre”
shares these facts with us in an article published 26 October 1998
(Ref 98/251, KEEPING MILK FRESHER, LONGER):

“When milk spoils before its use-by date, even when it has been
carefully stored and handled, it is usually because of the presence of
a group of cold-loving microbes called psychrotrophs, says Food
Science Australia dairy researcher Dr Heather Craven. "In health terms
these bacteria are pretty harmless, but they do pose a problem if they
get into the milk because then whole batches can go off - and dairy
companies receive complaints from consumers," she explains. "Even if
you keep the milk chilled, they can multiply from a few per millilitre
to over a million in the space of just six days. Even one microbe in a
carton of milk can cause a spoilage problem."

Thus the 6-day window. Although milk can remain edible far beyond the
expiration date in some instances, this is the earliest stage at which
the milk might become unsafe to consume. Other factors that affect the
freshness of the milk is the temperature at which it is stored and
improper handling, such as drinking directly from the carton.

But, alas, you are looking for a method and not a lesson. The long
held method of telling whether or not milk is spoiled is the look and
sniff method. If the milk has an unpleasant smell and/or if there is
any separation, the milk is considered by most to be spoiled. This
however is a matter of interpretation. Living in the US south, (and
for my sake I hope you have a sense of humor, because, after all, this
was my life experience) as a child I can recall my grandmother
sniffing the milk and calling it “blinky”. Blinky milk was not served
to drink because it “may” be bad, but it was not thrown out either.
Instead it was used for making breads such as biscuits or cornbread,
both of which cook at very high temperatures that assured the
elimination of potentially dangerous bacteria. Now, I don’t know where
you are from and you may in fact think all this is rather humorous,
but different cultures see things in different ways; so you see, what
might be spoiled to you might be considered fine cooking ingredients
to others. If grandma said the milk was “curdled” (a southern form of
the word “curd”, as in cottage cheese) on the other hand, this meant
that it was sour and had not only separated but that the solids had
reformed in the bottom of the container as curds. This milk was
immediately discarded to the animals, as it was no longer considered
safe for human consumption. This was the way I was taught and I use
her method to this day as a means of “testing” the milk for my own
family's sake.

To answer your question: While there is no commercial litmus test or
hand-dandy test kit that you can buy in order to test your milk for
dangerous microbes, the AFISC (mentioned above) is currently working
on a project called the “PsychroFast” milk test that is already being
used by milk producers in Australia may, at some point become
available on the commercial market. If you are asking this question
because you had the brilliant idea that such a device on the inside of
a milk carton which would turn a certain color when the milk went bad
would net you millions of dollars, it’s a great idea, but not a new
one. If you invent it and put it on the market however, that “will” be
something new since it appears that none of the great minds before you
ever actually followed through on the concept; at least not on a
successful mass scale. Heck, as proficient as I am with grandma’s
method, I might even give it a try myself.

Below you will find that I have carefully defined my search strategy
for you in the event that you need to search for more information. By
following the same type of searches that I did you may be able to
enhance the research I have provided even further. I hope you find
that that my research exceeds your expectations. If you have any
questions about my research please post a clarification request prior
to rating the answer. Otherwise, I welcome your rating and your final
comments and I look forward to working with you again in the near
future. Thank you for bringing your question to us.

Best regards;
Tutuzdad-ga


INFORMATION SOURCES

INNOVATIONS STORIES
http://www.abc.net.au/ra/elp/innovatn/inots710_a.htm

CSIRO MEDIA RELEASE
http://www.dfst.csiro.au/pr/mr98251.html

C SCIENCE
(This is in some other language but gives a visual example of how the
test might work)
http://www.c-science.com/txt/tc/te/ot/981213teotx.htm

CSIRO (Australia's “Commonwealth Scientific and Industrial Research
Organisation”)
http://www.csiro.au/




SEARCH STRATEGY


SEARCH ENGINE USED:

Google ://www.google.com


SEARCH TERMS USED:

Milk spoilage indicators

Milk spoilage indications

Milk spoilage indicated

Milk spoilage test

Milk spoiled test

“How to tell when milk is spoiled”
Comments  
Subject: Re: Milk Spoilage Detection
From: bobbie7-ga on 18 Jan 2003 13:02 PST
 
I’m not sure if this is what you’re looking for so I am posting this
information as a comment.

Methylene blue dye-reduction test: 
 
When the dye methylene blue or resazurin is added to milk, the
bacteria present take up oxygen and change the colour of the dye;
methylene blue loses its colour; resazurin changes from blue-purple to
pink. The speed of the change indicates the bacterial content.
Pasteurized milk must not reduce dye in less than half an hour.

Soure: Dictionary of Food and Nutrition 
http://www.xrefer.com/entry/536876

Methylene Blue Test 

Measuring the bacterial content of milk with the methylene blue test
is just one procedure scientists use to monitor the quality of our
foods. The blue dye indicates the presence of dissolved oxygen in the
milk. Since growing bacteria also require this oxygen, the time it
takes to disappear conversely indicates the amount of bacteria
present.
http://www.stemnet.nf.ca/d6vsatf2000/s1/Project10/organquality.html
Subject: Re: Milk Spoilage Detection
From: impostervt-ga on 18 Jan 2003 14:56 PST
 
Thanks for the comment. What I need to know though, is there a way to
perform a similar test without contaminating the milk? For instance,
given a gallon of milk is there a way to detect the bacteria levels in
the milk based on any gases the bacteria may be emiting that can be
detected.

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