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Q: Human Genome Project ( Answered 5 out of 5 stars,   0 Comments )
Question  
Subject: Human Genome Project
Category: Science > Biology
Asked by: rai130-ga
List Price: $2.00
Posted: 08 Nov 2004 02:13 PST
Expires: 08 Dec 2004 02:13 PST
Question ID: 426036
When scientists recently decoded the human genome, whose genetic
material did they use? Or maybe it didn't quite work like that...
Answer  
Subject: Re: Human Genome Project
Answered By: answerfinder-ga on 08 Nov 2004 03:16 PST
Rated:5 out of 5 stars
 
Dear rai130-ga,

According to Department of Energy (Oak Ridge National Laboratory) web
page on the Human Genome Project, Facts About Genome Sequencing, the
genetic material came from donors whose identity was protected. A
newspaper report from 2000 states the US government used material from
13 individuals, while a private company used 5 individuals.

"...in the international public-sector Human Genome Project (HGP),
researchers collected blood (female) or sperm (male) samples from a
large number of donors. Only a few of many collected samples were
processed as DNA resources. Thus the donor identities were protected
so neither donors nor scientists could know whose DNA was sequenced.
DNA clones from many different libraries were used in the overall
project."
http://www.ornl.gov/sci/techresources/Human_Genome/faq/seqfacts.shtml

For additional information on the project, this is the home page.
http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml

This is corroboration of the above.

"Who is Involved in the Human Genome Project?  
The project's major participants come from government (notably the US
National Institutes of Health and the US Department of Energy),
academia, industry, and the nonprofit world (Wellcome Trust). There is
also a company that is competing to sequence the genome.

The other notable participants are the many men and women who provided
DNA to the project. Ultimately, the DNA of 13 individuals was used
[1]. Their names are protected such that neither the scientists nor
the donors know whose DNA is actually being sequenced. The results of
the Human Genome Project will be applicable to all humans, however,
because the DNA differences between us are extremely small
statistically."
http://health.discovery.com/minisites/dna/zb_genome.html


The National Human Genome Research Institute provided guidelines on
the use of DNA in sequencing (issued in 1996).

"The Use of Human Subjects in Large-Scale DNA Sequencing
NHGRI-DOE Guidance on Human Subjects Issues in Large-Scale DNA Sequencing

...There are a number of approaches to preventing a DNA donor from
knowing that his/her DNA was actually sequenced as part of the HGP.
For example, each time a clone library is to be made, an appropriately
diverse pool of between five and ten volunteers can be chosen in such
a way that none of them knows the identity of anyone else in the pool.
Samples for DNA preparation and for preparation of a cell line can be
collected from all of the volunteers (who have been told that their
specimen may or may not eventually be used for DNA sequencing) and one
of those samples is randomly and blindly selected as the source
actually used for library construction. In this way, not only will the
identity of the individual whose DNA is chosen not be known to the
investigators, but that individual will also not be sure that s/he is
the actual source."
http://www.genome.gov/10000921

National Human Genome Research Institute home page
http://www.genome.gov/


Detroit Free Press
"Celera based its research on five people's white blood cells: two
white men, one black woman, one Asian woman, and one Hispanic woman.
The government used 13 people."
http://www.freep.com/news/nw/gene27_20000627.htm


I hope this answers your question. If it does not, or the answer is
unclear, then please ask for clarification of this research before
rating the answer. I shall respond to the clarification request as
soon as I receive it.
Thank you
answerfinder

Search strategy
"human genome project" whose dna
://www.google.com/search?hl=en&q=%22human+genome+project%22+whose+dna&spell=1
rai130-ga rated this answer:5 out of 5 stars
Most impressive as usual...

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